Cloning Vectors - Plasmid, Bacteriophage - Unsolved Practice Set
Chapter: Biotechnology Principles and Processes | Topic: Cloning Vectors Plasmid Bacteriophage
CLONING VECTORS - PLASMID, BACTERIOPHAGE - UNSOLVED PRACTICE SET
Topic: Cloning Vectors Plasmid Bacteriophage
Multiple Choice Questions
Q1. A cloning vector is best described as:
- A DNA molecule that can carry a foreign DNA fragment into a host cell and replicate within it
- An enzyme that cuts DNA
- A type of host cell only
- A protein used in gene expression
Q2. Which of the following is an essential feature of a good cloning vector?
- A large, complex size
- An origin of replication, allowing the vector to replicate independently within the host
- The complete absence of any selectable marker
- Inability to be introduced into a host cell
Q3. Plasmids used as cloning vectors are best described as:
- Part of the main bacterial chromosome
- Small, circular, extra-chromosomal, self-replicating DNA molecules found in bacteria
- A type of virus only
- A type of restriction enzyme
Q4. pBR322 is a classic and widely used example of a:
- Bacteriophage vector
- Plasmid cloning vector
- Restriction enzyme
- Selectable marker gene
Q5. Besides plasmids, another commonly used type of cloning vector, derived from a virus that infects bacteria, is:
- A restriction enzyme
- A bacteriophage
- A ribosome
- A polymerase enzyme
Short Answer Questions
Q6. Define a cloning vector, and explain its general role in recombinant DNA technology.
Q7. Name any two essential features that a good cloning vector should possess.
Q8. What are plasmids, and why are they commonly used as cloning vectors?
Q9. What is a bacteriophage-based vector, and how does it differ from a plasmid vector?
Long Answer Questions
Q10. Discuss the essential features required for an effective cloning vector, explaining why each feature is important for successful gene cloning.
Q11. Compare plasmid vectors and bacteriophage vectors, describing their basic characteristics and their respective roles in recombinant DNA technology.
APPLICATION / ANALYSIS
Q12. A biotechnology student is designing a cloning experiment and needs to choose a vector with a convenient site for inserting a gene of interest without disrupting the vector's ability to replicate or be selected afterward. Explain what specific vector feature would help the student achieve this.
Q13. A researcher compares using a plasmid vector versus a bacteriophage vector for cloning a relatively large piece of foreign DNA. Considering typical carrying capacities, suggest which vector type might be more suitable for cloning larger DNA fragments, and explain your reasoning.