Recombinant DNA Technology - Steps - Unsolved Practice Set
Chapter: Biotechnology Principles and Processes | Topic: Recombinant DNA Technology Steps
RECOMBINANT DNA TECHNOLOGY - STEPS - UNSOLVED PRACTICE SET
Topic: Recombinant DNA Technology Steps
Multiple Choice Questions
Q1. Recombinant DNA technology refers to techniques used to:
- Combine DNA from two different sources to create new genetic combinations
- Completely destroy DNA molecules
- Prevent all gene expression
- Only sequence existing DNA without modification
Q2. The first step in a typical recombinant DNA technology procedure is generally:
- Insertion of DNA into a host cell
- Isolation of the desired DNA (genetic material)
- Selection of transformed cells
- Downstream processing
Q3. After the desired gene and vector DNA have both been cut with a suitable restriction enzyme, the next step typically involves:
- Joining (ligating) the gene of interest with the vector DNA
- Discarding the vector DNA
- Directly injecting DNA into a human patient
- Skipping to downstream processing
Q4. The process of introducing recombinant DNA into a suitable host cell is called:
- Ligation
- Transformation
- Restriction digestion
- Gel electrophoresis
Q5. Once a desired protein product has been produced by genetically modified host cells, the process of separating and purifying it is called:
- Upstream processing
- Downstream processing
- Restriction digestion
- Transformation
Short Answer Questions
Q6. List the general steps involved in a typical recombinant DNA technology procedure, in the correct order.
Q7. What is meant by "ligation" in the context of recombinant DNA technology?
Q8. Define transformation, and explain why it is a crucial step in recombinant DNA technology.
Q9. Why is downstream processing necessary after a genetically modified organism has produced the desired product?
Long Answer Questions
Q10. Describe, step by step, the overall process of recombinant DNA technology, from isolating the desired gene to obtaining a purified final product.
Q11. Explain the significance of each major step in recombinant DNA technology, discussing why skipping or improperly performing any one step could affect the success of the overall process.
APPLICATION / ANALYSIS
Q12. A biotechnology student is asked to summarise the entire process of producing recombinant human insulin, from isolating the human insulin gene to obtaining the final purified insulin product. Outline the sequence of major steps the student should include in this summary.
Q13. A research team successfully inserts a gene of interest into a plasmid vector and transforms bacterial host cells with it, but does not include any method for selecting only the successfully transformed cells afterward. Explain, using your understanding of recombinant DNA technology, why this omission could create a significant practical problem.