Transcription in Prokaryotes - Unsolved Practice Set
Chapter: Molecular Basis of Inheritance | Topic: Transcription in Prokaryotes
TRANSCRIPTION IN PROKARYOTES - UNSOLVED PRACTICE SET
Topic: Transcription in Prokaryotes
Multiple Choice Questions
Q1. Transcription is the process by which:
- DNA is copied into more DNA
- A single-stranded RNA copy is synthesised from a DNA template
- Proteins are synthesised from mRNA
- DNA is packaged into chromatin
Q2. In prokaryotes, transcription is carried out by:
- Three different RNA polymerases
- A single type of RNA polymerase
- DNA polymerase only
- Ribosomes directly
Q3. The DNA strand that is actually used as a template for RNA synthesis during transcription is called the:
- Coding strand
- Template (antisense) strand
- Sense strand
- Leading strand
Q4. The specific DNA sequence to which RNA polymerase binds to initiate transcription is called the:
- Terminator
- Promoter
- Operator
- Enhancer
Q5. Termination of transcription in prokaryotes can occur through a mechanism that depends on a protein called:
- Sigma factor
- Rho factor
- Helicase
- Ligase
Short Answer Questions
Q6. Define transcription, and name the enzyme responsible for it.
Q7. Differentiate between the template strand and the coding strand of DNA during transcription.
Q8. What is a promoter, and what role does it play in transcription?
Q9. Briefly describe what happens during the termination stage of transcription in prokaryotes.
Long Answer Questions
Q10. Describe the three main stages of transcription - initiation, elongation, and termination - in prokaryotes.
Q11. Explain the structure of a typical transcription unit, describing the significance of the promoter and terminator regions.
APPLICATION / ANALYSIS
Q12. A researcher studying bacterial gene expression finds that a mutation in the promoter region of a gene significantly reduces the amount of mRNA produced from that gene. Explain, using your understanding of transcription, why a promoter mutation would have this effect.
Q13. If RNA polymerase in a bacterial cell were to mistakenly use the coding strand instead of the template strand for transcription, predict what kind of RNA molecule would be produced, and explain why this would be non-functional.