Downstream Processing - Unsolved Practice Set

Q1. Downstream processing refers to the series of processes carried out:

1. Before the biosynthesis of a product begins
2. After the biosynthesis stage, to separate and purify the desired product
3. Only during the transformation step 
4. Only during PCR amplification

Q2. One of the main purposes of downstream processing is to:

1. Introduce foreign DNA into a host cell
2. Separate and purify the desired product from the culture medium and other unwanted materials
3. Cut DNA at specific recognition sites
4. Amplify a specific DNA sequence

Q3. Before a biotechnologically produced pharmaceutical product can be marketed, it typically must undergo:

1. No further testing at all
2. Rigorous quality control testing to ensure safety and efficacy
3. Only visual inspection
4. Testing only for colour and packaging

Q4. Formulation, as part of downstream processing, generally involves:

1. Only separating the product from the culture broth
2. Adding suitable preservatives and preparing the product in a form suitable for its intended use
3. Cutting the DNA of the host organism
4. Introducing the vector into a new host cell

Q5. Downstream processing is considered an essential part of the biotechnology production process mainly because:

1. It has no real impact on the final product
2. It ensures that the final product is sufficiently pure, safe, and suitable for its intended use before reaching the market
3. It replaces the need for a bioreactor entirely
4. It occurs before any biosynthesis takes place